Optolong optics Ltd

Achievement: Fluorescence test image with by FITC filter
Photographed by Daniel Han.
Images were captured by single band
DAPI(30024):EX365,EM445,DM405.
Taken by Olympus microscope and Nikon camera.
Filter sets: Optolong fluorescence filters.

The image acquired by Optolong dual bandpass filter sets Green/Orange with a 20x objective, Nikon microscopy Ni-U.
🔬👩‍🔬 Scientific artwork by Optolong filter distributor.

Image of lichens!

Photographer👨‍🔬: Frantisek Bednar
Filter Sets🔍: DAPI longpass filter set
(Excitation BP365, Emission LP415, Dichroic 405)
Microscope🔬: microscope

Achievement: Fluorescence test image with by FITC filter
Photographed by Daniel Han.
Images were captured by single band (30025).
Taken with Olympus microscope and Nikon camera.

Image of "Convallaria Stem Section"

Photographer👨‍🔬: Bednar
Filter set🔍️: LP-DAPI PN31001
(Excitation BP365nm, Emission LP415nm, Dichroic 405nm)
Microscope🔬: BX51
Specimen🥀: Acridin stained the Convallaria stem section

Achievement: Fluorescence test image with by long pass DAPI filter
Photographed by Frantisek Bednar.
Images were captured by
long pass band (31001)

It was taken with an Olympus BX51 microscope with BX-RFA DIC and UPLSAPO objectives, and Canon EOS R7, Acridin stained the Convallaria stem section. Low-resolution results attached (4x and 20x).

Filter sets: Optolong fluorescence filters

Why Do We Need Filters In Microscopy? Microscopy filters act to modify the light within an optical imaging system. This can be for observation purposes or for capturing high-quality images using a detector.
Highly effective fluorescence filter sets are necessary to provide maximum attenuation of excitation light in the detection pathway, in parallel with the successful capture of as many emitted photons as possible.
The image was acquired with Optolong dual bandpass filter sets Green/Orange with a 20x objective, Nikon microscopy Ni-U.
🔬👩‍🔬 Scientific artwork by Optolong filter distributor, China.

Happy International Tea Day! Did you know that FTIR microscopy is revolutionizing quality control in the tea industry?

Fluorescence filter sets are critical for microscopy that modify the light within an optical imaging system. This can be for observation purposes or for capturing high-quality images using a detector. Each microscopy filter can serve a different purpose and filters can be used for various improvements such as: improving contrast and signal-to-noise ratio, resulting in precise and dependable imaging.

The image was acquired with Optolong dual bandpass filter sets Green/Orange with a 20x objective, Nikon microscopy Ni-U.

🔬👩‍🔬 Scientific artwork by Optolong filter distributor, China.

Achievement: Fluorescence test image with by FITC filter
Photographed by Daniel Han.
Images were captured by single band
(30025),DAPI(30024),FISH-ORANGE(30033)
Taken with Olympus microscope and Nikon camera.
Filter sets: Optolong fluorescence filters

More of interesting and stunning images acquired by

*Single-band filter sets DAPI, FITC, TRITC,TexasRed, Cy5,Cy7,
*Longpass DAPI,FITC,TRITC,TexasRed
*Multi-band filter sets
All of these filters are available at Optolong.

Achievement: Fluorescence test image with by FITC filter
Photographed by Daniel Han.
Images were captured by single band (30025).
Taken with Olympus microscope and Nikon camera.

Do you know what this cell sample is?
In biology, the smallest unit that can live on its own and that makes up all living organisms and the tissues of the body. A cell has three main parts: the cell membrane, the nucleus, and the cytoplasm. I got this tested image from Daniel that was taken with FITC and FISH-Orange merged.
The specification of these two filter sets:
PN30025 FITC:EX:475,EM:530,DM:500;PN30033 FISH-Orange:EX540,
EM590,DM562nm;
Unfortunately, we don't know what this cell's name is, could someone know this?

What? Did we improve the resolution of the images? We don't have those capabilities yet, so took a Z-Stacked photo of one of the microphone holes of a . What you see in is dirt in using the filter dual-band 32012 from optolongfluorescencefilter
Photographer: Química Tech SA de CV
Filter sets: Optolong Dual-band Green/Orange filter

What? Did we improve the resolution of the images? We don't have those capabilities yet, so took a Z-Stacked photo of one of the microphone holes of a . What you see in is dirt in using the filter dual-band 32012 from optolongfluorescencefilter

Photographer: Química Tech SA de CV
Filter sets: Optolong Dual-band Green/Orange filter

When your teacher tells you that you have to learn the basics is because you need to learn the basics... not only because you the basics in order to master your skills but also to save money..
These are images of pulmonary cells where the is stained with DAPI, the cytoskeleton with Alexa488, and the mitochondrion with Mitotracker Red. In the first image we see on top the combined channels using filter sets FISH AQUA + Dual band green orange, both illuminated with Vis LED lamp.
The lower one is the same sample but with filter sets DAPI LONGPASS + Dual band green orange and an HBO50w lamp. The results are pretty much the same, the objective is to contrast the nucleus, cytoskeleton, and mitochondrion. Which one do like the most? And which one is the cheapest?

Photographer: Química Tech SA de CV
Filter sets: Optolong FISHAqua and Dual-band Green/Orange filter

This is a filter (used for technical cleanliness analysis ) in with dual-band filter set green/orange PN32012.
Photographer: Química Tech SA de CV
Filter sets: Optolong Dual-band Green/Orange filter

What is the difference between IBS, IAD, and IBAD?
IAD coatings are widely utilized for optical systems designed for the defense industry where multiple laser wavelength devices are required to work together in rugged environments. Ion beam sputtering (IBS) generates very dense, hard coatings ideal for high reflection and low loss requirements. IBAD is widely used for top-quality optical coatings that require exact tuning of the reflective index with the density of the films.
Optolong professionally manufactures optical thin film with Vacuum ion-assisted coating(IBAD), Bombarding a growing film with an ion beam greatly enhances several critical performance factors including density, hardness, and adhesion with greater control of surface texture and microstructures. better mechanical durability and environmental stability, being resistant to moisture and weathering. The thin film deposition process for many applications requires precise deposition thickness and high control of film properties such as density, bonding, and durability.

This is a single-band filter set TexasRed, with wider excitation and emission bandpass windows that allow it to transmit more energy and produce comparatively brighter images. Resulting images also reflect additional red fluorescence signals due to the extension of the emission filter passband to longer wavelengths in the red spectral region—high brightness and high contrast, coated on single-piece transparent glass with low auto-fluorescence.

High Contrast DAPI excitation emission filter set in the life science field, as it can pass through an intact cell membrane, it can be used to stain both live and fixed cells, optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Optolong used a hard-coated exciter working well under intense UV illumination of modern fluorescence light sources.
Contain individual filters: EX 365nm, DM 405nm, EM 445nm.
Fluorophore Displayed: DAPI, AMCA (Aminomethylcoumarin), LysoSensor Blue, Alexa Fluor 350™, Marina Blue, Pacific Blue™, sgBFP™
Compatible Microscopes： most common Nikon, Olympus, Zeiss, Leica, and other brand microscopes.



Stable Hard Coatings, High contrast image, Optimized Optical fabrication for brilliant imaging, Extremely sharp transitions from rejection to transition regionsLowest Auto-fluorescence, Excellent set signal-to noise ratio, Superior field life.

PN 30027 TRITC (Tetramethylrhodamine-isothiocyanate) is a fluorescent compound with an excitation peak at 544 nm and an emission peak at 570 nm, which is designed to fit in standard filter cubes from the leading microscope brand, suitable for LED light sources.
As the emission band peaks in the orange, the fluorescence correctly appears orange. It is optimized for TRITC, SpectrumOrange， Propidium Iodide (PI)， Resorufin， Pro-Q Diamond， Merocyanine 540，mApple， LOLO-1， and ATTO 565.

Achievement: Fluorescence test image with Osmanthus fragrans stem by FITC filter
Photographed by Daniel Han.
Images were captured by single band (30025), (30033) .
Taken with Olympus microscope and Nikon camera.

Hela cells: FISH-Orange!
Photographed by Daniel Han.
Images were captured by narrow bandpass filter set, (30033) .
Taken with Olympus microscope and Nikon camera.

Hela cells: FITC and FISH-Orange merged!
Photographed by Han.
Images were captured by single band (30025), (30033) .
Taken with Olympus microscope and Nikon camera.

We are honored to be commented by one sole agent of the top 3 fluorescence filter suppliers in the US to be the NO.1 supplier who makes fluorescence filters in China.
Warranty Information for Optolong fluorescence filters:
1) Hard coating
2) High transmittance T＞90%
3) Deep blocking OD＞6
4) Coated on single piece transparent glass, low auto-fluorescence
5) High-brightness, high-contrast
6) Long years warranty

BPAE cells !
Photographed by Brian Matsumoto .
Images were captured by single band (30024) , (30025), (30027) .
Details of the filter sets:
DAPI : EX365/50 , EM445/50 , DM405
FITC: EX475/40 , EM530/50 , DM500
TRITC: EX545/30, EM605/40 , DM575

Monday Achievement: Fluorescence test image with bulb pulp in pear taken by FISH-Orange.

Image credit👨‍🔬: Daniel Han
Filter set🔎: Narrow band FISH-Orange filter set PN30033
Equipment 🔬: Olympus inverted microscope with light engine, 40x, pear sclereids, stained with Rhodamine and Acridine Orange, Optolong FISH-Orange PN30033.

Image of Nuclei, stained with DAPI.
The filter set is narrow band DAPI filters from .

Image credit👨‍🔬: Daniel Han
Filter set🔎: Narrow band DAPI filter set
Microscope 🔬: IX73

We are planning to increase investment in manpower, logistics, product research, and development in 2024, we can produce a thousand filters per week for all industry companies, sincerely hope to have a very fruitful 2024 together with all amateur and professional microscopists, scientists, physicians, clinicians and experts teams.

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