KIMIKA: The Journal of the Kapisanang Kimika ng Pilipinas

KIMIKA: An Open Access Journal
Vol. 32 No. 2 (2021)

Extraction of Milled Wood Lignin from Coffee Husk (Coffea Arabica L.) and the Analysis of Its Potential as a UV-Protective Component of Lotion and Sunscreen

Christian John Cordero
Institution of Chemistry, College of Arts and Science, University of the Philippines Los Baños, College, Laguna, 4030
https://orcid.org/0000-0002-7707-5831

Kevinilo Marquez
Institution of Chemistry, College of Arts and Science, University of the Philippines Los Baños, College, Laguna, 4030
https://orcid.org/0000-0002-5745-464X

Keywords: Björkman method, Mansur method, Milled wood lignin, sun protection factor

Abstract

This study aims to determine the effect of adding milled wood lignin (MWL) to the sun protection factor (SPF) of commercially available lotion and sunscreen. The MWL was isolated from Coffea Arabica L. husks using the Björkman method. FT-IR spectroscopic analysis showed two characteristic peaks located at around 1200 cm-1 and 1300 cm-1, which indicates the presence of guaiacyl and syringyl groups. UV-Vis spectroscopic analysis showed two peaks located at around 278 nm and 325 nm, which indicates the presence of guaiacyl and some conjugated phenolic groups, respectively. The isolated MWL was then added to commercially available sunscreen and lotion at various concentrations. The sun protection factor (SPF) was then determined using the Mansur method and UV-Vis Spectroscopy. Results have shown that from 0% to 40 % by mass of MWL added, there was an observable average increase from 0.90 to 13.86 SPF for lotion, while there was an increase from 26.47 to 34.00 SPF for sunscreen. Results suggest that for lotion the SPF significantly increased if MWL was added from 0% up to 40% while for sunscreen the SPF significantly increased if MWL was added from 0% up to 20%.

https://kimika.pfcs.org.ph/index.php/kimika/article/view/339

KIMIKA: An Open Access Journal
No Publication Fees

Rapid-Throughput Analysis of Human Milk Oligosaccharides from Filipino Breastmilk

Anthony Salvador Albaladejo
Department of Chemistry, University of the Philippines Visayas, Miagao, Iloilo 5023
https://orcid.org/0000-0002-1216-8359

Gladys Cherisse Completo
Institute of Chemistry, University of the Philippines Los Banos, Laguna 4031
https://orcid.org/0000-0002-7815-8954

Baby Richard Navarro
Institute of Food Science and Technology, University of the Philippines Los Banos, Laguna 4031
https://orcid.org/0000-0002-8854-7005

Ruel Nacario
Institute of Chemistry, University of the Philippines Los Banos, Laguna 4031

Carlito Lebrilla
Department of Chemistry, and Biochemistry (School of Medicine), University of California, Davis, CA 95616

Keywords: Human milk oligosaccharides, Rapid-throughput, Nano-HPLC chip/TOF-MS

Abstract
Human milk oligosaccharides (HMOs), although considered indigestible to the infant, are the third most abundant solid component of breastmilk. They serve many health-related functions benefitting the infant, such as nourishment of gut bacteria, inhibition of pathogenic infection, and aiding in brain development. In this study, a rapid-throughput analytical method is validated for Filipino breastmilk HMOs. The method entails lipid extraction by centrifugation, protein precipitation using ethanol, alditol sugar reduction using sodium borohydride, and solid phase extraction purification using porous graphitized carbon, all in a 96-well plate format. Nano-HPLC Chip/TOF-MS, coupled with the use of Agilent MassHunter programs for filtering datasets using an in-house library, is then used to analyze purified HMOs. The results evidently show that the rapid-throughput method minimizes sample loss, ensures reproducible MS signals, and considerably shortens sample handling time. Furthermore, the method significantly reduced analysis time 5-fold and eliminated the individual sample preparation, which enabled simultaneous analysis of 386 samples (four 96-well plates) in a single run. Thus, the rapid-throughput analysis is highly efficient and effective for the profiling and structural determination of Filipino breastmilk HMOs even in large sample population sets.

https://kimika.pfcs.org.ph/index.php/kimika/article/view/340

KIMIKA: Open Access Journal
Vol. 32 No. 2 (2021)

A New Pentacyclic Triterpenoid from Albizia lebbekoides (DC) Benth (Leguminosae)

Yohannes Woldemariam
Natural Products Research Laboratory, Institute of Chemistry, University of the Philippines, Diliman, Quezon City 1101

Irene Villaseñor
Natural Products Research Laboratory, Institute of Chemistry, University of the Philippines, Diliman, Quezon City 1101

https://orcid.org/0000-0003-0137-7822
DOI: https://doi.org/10.26534/kimika.v32i2.41-46

Abstract
Albizia lebbekoides (DC) Benth is one of the five species of the genus Albizia found in the Philippines. Not much is known about the chemistry and potential uses of this species. Solvent partition followed by sequential and repeated liquid chromatographic purification over silica led to the isolation of a compound from the hexane extract of A. lebbekoides leaves. A review of related literature and spectral analyses showed that the isolated compound is a new pentacyclic triterpenoid with an oleanane skeleton. Its structure was determined as 3β, 16α-dihydroxyolean-6(7),12(13)-dien-28-oic acid.

https://kimika.pfcs.org.ph/index.php/kimika/article/view/343

On her birthday today, we celebrate the life of Dr. Consolacion Y. Ragasa, one of the pillars of Natural Products Chemistry research in the country.

You can read about her life's work in an article published early this year in KIMIKA Volume 32 Number 1.

Link:
https://doi.org/10.26534/kimika.v32i1.58-69

In celebration of World Environment Day, today's featured article focuses on the adsorption of heavy metals in aqueous solution.

Authors from the Institute of Chemistry of the University of the Philippines Los Baños analyzed the Adsorbent Properties of Lignin extracted from Talahib (Saccharum spontaneum L.) towards heavy metals Cu(II) and Pb(II) in Aqueous Solution.

Download Link:
https://doi.org/10.26534/kimika.v32i1.46-57

'CHEMISTORY: Integration of Creative Story Writing in Understanding Chemical Elements in Online Learning' presents a pedagogical exploration of Junior high school teachers in drawing an integration between creative writing and chemistry concepts in understanding the behavior of chemical elements as means of addressing the competencies of the Science and English learning areas in an online instructional delivery.

Check out the article written by members of the University of Santo Tomas Junior High School Department in KIMIKA's latest volume.

Link:
https://kimika.pfcs.org.ph/index.php/kimika/article/view/335

The pandemic has provided an opportunity for chemistry educators to be resourceful, creative, and adaptive in communicating technical concepts to students. That said, teaching and honing chemistry laboratory skills is a continuous challenge.

This paper from the KIMIKA's latest volume focuses on determining the feasibility of conducting a laboratory experiment on natural acid-base indicators as a home-based activity.

Link:
https://kimika.pfcs.org.ph/index.php/kimika/article/view/325

KIMIKA Vol. 32 No. 1 (2021) is now available for download.

COVER:
Dr. Consolacion Y. Ragasa (1955-2021) in a DLSU University Fellow academic toga taken at De La Salle University in Taft Avenue, Manila, Philippines, circa 2011, behind the "Centennial" Tree, Barringtonia asiatica, which she extensively studied for its chemical constituents.

📷: Nikki Andrei V. Tan

https://kimika.pfcs.org.ph/index.php/kimika/issue/view/41

KIMIKA is the professional journal of the Kapisanang Kimika ng Pilipinas, Inc. (KKP). It publishes results of scientific research to serve and promote the chemistry profession and allied fields. It publishes peer-reviewed works on the broad spectrum of chemistry research and chemistry education as full articles, short communications, reviews or opinions.

Official Website:
https://kimika.pfcs.org.ph

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