HPLC

ASES Cromatografía de Liquidos HPLC

🔵 Equipo en venta
▪️️Cromatografo de Liquidos Uv-Vis.
▪️Waters, Enpower3
▪️Venta en México.
▪️Cotizacion: [email protected]






HPLC

ASES Cromatografía de Liquidos HPLC

ww.aseshplc.com
[email protected]

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HPLC High performance liquid chromatography or High pressure Liquid chromatography is an analytical instrument, used to identifying, Quantify...

What is the HPLC function in your Job?
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HOW TO BE MORE SUCCESSFUL
WITH HPLC ANALYSIS: PRACTICAL
ASPECTS IN HPLC OPERATION

Filtration :
Aqueous mobile phase containing buffers or ion-pairing reagents must be filtered. However, when a high-purity buffer (>99.99%) is
used in performing trace analysis such as impurity testing, the filtration step might be skipped to reduce potential solvent contaminations that lead to gradient peaks. Do not filter HPLC-grade solvents, which are
typically pre-filtered by the manufacturers. Filter all organic or aqueous solvents through a 0.45 um membrane filter (typically 47-mm diameter). Use cellulose acetate filters for aqueous solvents, PTFE filters for organic
solvents, and nylon filters for either organic or aqueous solvents.

High Performance Liquid Chromatography HPLC- UV-VIS Detector Animation I make animations in biology with PowerPoint, this animation video is about High-performance liquid chromatography, which is a technique in analytical chemis...

HOW TO BE MORE SUCCESSFUL
WITH HPLC ANALYSIS: PRACTICAL
ASPECTS IN HPLC OPERATION

Buffers :
Buffers are commonly used to control the pH of the mobile phase, which greatly affects the retention of acidic or basic analytes. Any pH adjustments should be made before mixing the aqueous buffer with any organic solvents. Precaution should be taken to prevent any possibility of buffer precipitation. While methanol is fairly miscible with most buffers, acetonitrile might precipitate some buffers at high concentrations (e.g., above 60-80%). Note that lower buffer strengths have fewer problems for buffer precipitation as well as substantially less background in HPLC applications. An acidic pH of 2.5-3 is a good starting point for most pharmaceutical applications because the low pH suppresses the ionization of most acidic analytes (and provides a higher retention) and minimizes the interaction of basic analytes with surface silanols on the silica packing (because silanols are not ionized at that pH).

Automatic Titrator COM-1700A

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https://youtu.be/K0p8XmYVqc0

HIRANUMA Automatic Titrator COM-1700A COM-1700A System Configuration and Example of Neutralization Titration. http://hiranuma.com/english/products/titr/com-1700a/com-1700a.html

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