CELLEC Biotek AG

In this paper, a new concept of a structural scaffold design is presented where the physiological multi-scale architecture is fully incorporated in a single-scaffold solution. Hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) bioceramic scaffolds with different bioinspired porosity, mimicking the spongy and cortical bone tissue, were studied. In vitro experiments looking at the mesenchymal stem cells' behavior were conducted in a perfusion bioreactor that mimics the physiological conditions in terms of interstitial fluid flow and associated induced shear stress.

Bone-like ceramic scaffolds designed with bioinspired porosity induce a different stem cell response - Journal of Materials Science: Materials in Medicine Biomaterial science increasingly seeks more biomimetic scaffolds that functionally augment the native bone tissue. In this paper, a new concept of a structural scaffold design is presented where the physiological multi-scale architecture is fully incorporated in a single-scaffold solution. Hydroxyap...

Congratulation to Zihe Huo, Dr. Stephanie Gros, and all co-authors for publishing their new work!

The authors generated a microtissue by co-culturing BT474 and 3T3 cells on a collagen scaffold in the U-CUP perfusion bioreactor device and studied the ability of doxorubicin nanodrug to accumulate in such a complex tumor model.

Transforming the Chemical Structure and Bio‐Nano Activity of Doxorubicin by Ultrasound for Selective Killing of Cancer Cells The ultrasonic transformation of doxorubicin into a tumor selective nanodrug is presented. The nanodrug maintains the antiproliferative activity against several cancer cell lines without being toxic ...

Congratulation to Maria Adelaide Asnaghi and colleagues for the recent publication in Advanced Functional Materials journal. https://doi.org/10.1002/adfm.202010747
The authors developed a decellularization procedure in perfusion bioreactor that allows efficient removal of cells and nuclear matter, while preserving extracellular matrix native architecture and protein composition.

Thymus Extracellular Matrix‐Derived Scaffolds Support Graft‐Resident Thymopoiesis and Long‐Term In Vitro Culture of Adult Thymic Epithelial Cells A novel 3D scaffold derived from the thymic extracellular matrix seeded in vitro by either fetal or adult thymic epithelial cells (TEC) supports thymopoiesis over an extended period of time. The succ...

Swiss Society for Biomaterials and Regenerative Medicine (SSB+RM) 4th Young Scientist Annual Symposium

Date: Thursday, October 7th, 2021
Location: ETH Hauptgebäude, Audi Max

Keynote speakers: Prof. Inge Herrmann and Prof. Olivier Guenat
https://ssbrm.ch/ssbrm2021ys/

Congratulation to Andreja Vukasovic and Maria Adelaide Asnaghi and colleagues for the recent publication. Another step towards implementing the bioreactor‐based manufacturing system for the production of human cartilage grafts for clinical applications.

Bioreactor‐manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies Objectives Bioreactor‐based production systems have the potential to overcome limitations associated with conventional tissue engineering manufacturing methods, facilitating regulatory compliant and...

Congratulation to Jenna Harvestine and colleagues for the recent publication (Leach Lab, UC Davis).
In this work, the authors perfused cells on ECM-coated scaffolds to generate naïve and preconditioned constructs. They demonstrated that bioreactor-based preconditioning augments the bone-forming potential of unfractionated bone marrow aspirate.

Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates Cell-derived extracellular matrix (ECM) provides a niche to promote osteogenic differentiation, cell adhesion, survival, and trophic factor secretion. To determine whether osteogenic preconditioning would improve the bone-forming potential of unfractionated bone marrow aspirate (BMA), we perfused ce...

Taking Patients Out of Medical Research

bloomberg.com Switzerland's Cellec Biotek provides researchers with the tools to grow artificial samples for medical research. Globalive Chairman Anthony Lacavera talks to Ivan Martin, research group leader. (Source: Bloomberg)

Perfusion bioreactor-based cryopreservation of 3D human mesenchymal stromal cell tissue grafts

In this work, the Authors demonstrated that addition and removal of cryoprotective agents under perfused flow significantly increased the viability of MSC within cryopreserved 3D tissue constructs as compared to conventional diffusion-based methods.
https://www.sciencedirect.com/science/article/pii/S0011224017300755?via%3Dihub #!

sciencedirect.com The generation of an off-the-shelf in vitro engineered living tissue graft will likely require cryopreservation. However, the efficient addition and r…

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twitter.com The latest Tweets from Cellec Biotek AG (): "The U-CUP is a user-friendly tool to establish and control your . U-CUP has been designed to be used by any lab member working in the related field, without requiring any previous experienc...

Hydrogen sulfide-releasing silk fibroin scaffold for bone tissue engineering

Congratulation to Dr. Grassi (IRCCS Istituto Ortopedico Rizzoli, Bologna) and his group for this work in which they evaluated the use of Hydrogen sulfide-releasing silk fibroin scaffolds for bone healing and regeneration applications, using the U-CUP Perfusion Bioreactor to seed, expand and differentiate hMSCs
https://www.sciencedirect.com/science/article/pii/S0928493118323580?via%3Dihub

sciencedirect.com Hydrogen sulfide (H2S)-based therapy is a promising therapeutic strategy for several biomedical applications. Following the observation that endogenou…

A perfusion bioreactor-based 3D model of the subarachnoid space based on a meningeal tissue construct

To overcome the lack of suitable in vitro models that faithfully recapitulate the intricate three-dimensional architecture, complex cellular interactions, and fluid dynamics within the subarachnoid space, the Authors (Corina Kohler) have developed a perfusion bioreactor-based 3D in vitro model using primary human meningothelial cells to generate meningeal tissue constructs
https://fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-019-0137-6

fluidsbarrierscns.biomedcentral.com

Human Mesenchymal Stem Cells Differentiation Regulated by Hydroxyapatite Content within Chitosan-Based Scaffolds under Perfusion Conditions

In Rogina et al. 2017, the authors investigated the influence of a different scaffold’s composition on the human hMSCs differentiation performed in a U-CUP bioreactor under perfusion conditioning

https://www.mdpi.com/2073-4360/9/9/387

mdpi.com The extensive need for hard tissue substituent greatly motivates development of suitable allogeneic grafts for therapeutic recreation. Different calcium phosphate phases have been accepted as scaffold’s components with positive influence on osteoinduction and differentiation of human mesenchymal s...

Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells

Perfusion culture of SVF-cells reproducibly promoted in vitro the early formation of a capillary-like network, embedded within an ECM backbone, and the release of numerous pro-angiogenic factors. Perfusion-based engineered constructs were more rapidly vascularized and supported a superior survival of delivered cells upon in vivo ectopic implantation.
https://www.nature.com/articles/s41598-017-13882-3

nature.com Article

Maintenance of Primary Human Colorectal Cancer Microenvironment Using a Perfusion Bioreactor‐Based 3D Culture System - Manfredonia - 2019 - Advanced Biosystems - Wiley Online Library

"Perfused cultures maintain tumor tissue architecture and densities of proliferating tumor cells to significantly higher extents than static cultures. Stromal and immune cells are also preserved and fully viable"

Find more U-CUP related publications here: https://bit.ly/2W4fB3s

onlinelibrary.wiley.com Full Paper Maintenance of Primary Human Colorectal Cancer Microenvironment Using a Perfusion Bioreactor‐Based 3D Culture System Celeste Manfredonia https://orcid.org/0000-0002-0929-037X Cancer Immunotherapy, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 4031 Swi...

Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity. - PubMed - NCBI

"Direct perfusion of cells through the scaffold pores proved to be an effective means to seed cells with a highly homogeneous distribution, both radially and axially"

"The more uniform spatial distributions achieved by perfusion seeding as compared to static or spinner flask methods could allow reduced variations in nutrient and metabolic product concentrations, previously suggested to yield heterogeneous cell growth and activity, such that the resulting constructs may have a greater potential to generate uniform 3D tissues."

Wendt et al., BIOTECHNOLOGY AND BIOENGINEERING, VOL. 84, NO. 2, OCTOBER 20, 2003
https://www.ncbi.nlm.nih.gov/pubmed/12966577

ncbi.nlm.nih.gov Biotechnol Bioeng. 2003 Oct 20;84(2):205-14. Comparative Study; Research Support, Non-U.S. Gov't

The authors studied the formation of bone tissue under perfusion flow, using DEX-loaded RADA 16-I hydrogel characterized by efficient delivery of small molecules for sustained release applications.
Materials 2019, 12(6), 919; DOI:10.3390/ma12060919
https://www.ncbi.nlm.nih.gov/pubmed/30893951

CELLEC Biotek AG

25th Annual Meeting of the Swiss Society for Biomaterials and Regenerative Medicine (SSB+RM2019)
May 22-23, 2019
Location: FHNW Campus, Muttenz, Switzerland
https://ssbrm.ch/ssbrm2019/

Congratulation Jenna for the great work!
In this study, the authors hypothesized that cell-secreted ECM could be used to sequester MSCs and accessory cells from bone marrow aspirate for bone regeneration and they used the U-CUP perfusion bioreactor system to generate 3D implantable constructs
https://www.ncbi.nlm.nih.gov/pubmed/30869662

Schimke et al. have explored the potential application of a poly-L-lactic acid (PLLA) and poly-ε-caprolactone (PCL) based scaffold for cell cultivation in 3D and facial reconstruction after trauma or tumor resection
Publications list: http://www.cellecbiotek.com/pages.aspx?lang=en&id=83&pnm=%201%204%2010%2083%20204

Lovely and creative people working with us :D Happy Valentines!

In this study by Foglietta et al., the authors described the preparation of keratin-based nanoparticles loaded with paclitaxel and their in vitro anticancer activity in 2D and perfusion 3D breast cancer models.
https://doi.org/10.2147/IJN.S159942

In this study, the authors describe the novel use of a bi-directional perfusion bioreactor to support the long term culture of human bone marrow stromal cells (BMSCs) differentiated on polylactic co-glycolic acid (PLGA)
http://bio.biologists.org/content/7/12/bio034488.long

SSB+RM2019 | SSB+RM

25th Annual Meeting of the Swiss Society for Biomaterials and Regenerative Medicine (SSB+RM2019)
May 22-23, 2019
Location: FHNW Campus, Muttenz, Switzerland
https://ssbrm.ch/ssbrm2019/

ssbrm.ch 25th Annual Meeting of the Swiss Society for Biomaterials and Regenerative Medicine (SSB+RM2019) May 22-23, 2019 Conference Location University of Applied Sciences and Arts Northwestern Switzerland FHNW, Muttenz, Switzerland The 1.5 day conference is open to everybody interested in the scientific, t...

In this study, the authors showed that dynamic cultivation of a novel porous oxygen plasma treated (OPT) HA scaffold with hBMSCs in osteogenic medium and subsequent decellularization provides a promising off-the-shelf bone tissue-engineered construct
https://goo.gl/kzzE6d

In this study, the authors suggested a potential new strategy for engineering osteogenic grafts with a mature ECM by modulating crosslink formation.
https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201700763

Decoration of RGD-mimetic porous scaffolds with engineered and devitalized extracellular matrix for adipose tissue regeneration

In this study, the authors combined the functionalities of a synthetic polymer with those of an engineered and subsequently devitalized extracellular matrix (ECM) to generate a hybrid material for adipose tissue regeneration

sciencedirect.com Fat grafting is emerging as a promising alternative to silicon implants in breast reconstruction surgery. Unfortunately, this approach does not provid…

Aortic valve cell seeding into decellularized animal pericardium by perfusion‐assisted bioreactor - Amadeo - 2018 - Journal of Tissue Engineering and Regenerative Medicine - Wiley Online Library

In the present contribution, the authors optimized a decellularization procedure that is permissive for seeding and culturing valve competent cells able to colonize and reconstitute a valve-like tissue

onlinelibrary.wiley.com Journal of Tissue Engineering and Regenerative Medicine Volume 12, Issue 6 RESEARCH ARTICLE Aortic valve cell seeding into decellularized animal pericardium by perfusion‐assisted bioreactor Francesco Amadeo Unità di Ingegneria Tissutale Cardiovascolare, Centro Cardiologico Monzino IRCCS, , Milan,...

Blood formation: Researchers engineer human bone marrow tissue

"The researchers have developed an artificial tissue that mimics some of the complex biological properties of natural bone marrow niches. To do this, they combined human mesenchymal stromal cells with a porous, bone-like 3D scaffold made of a ceramic material in what is known as a perfusion bioreactor, which was used to combine biological and synthetic materials."
Publication: Bourgine P.E., Klein T., et al., In vitro biomimetic engineering of a human hematopoietic niche with functional properties, PNAS June 4, 2018
https://www.pnas.org/content/115/25/E5688

eurekalert.org Researchers have developed an artificial tissue in which human blood stem cells remain functional for a prolonged period of time. Scientists from the University of Basel, University Hospital Basel, and ETH Zurich have reported their findings in the scientific journal PNAS.