डॉ. शिव मोहन सिंह

When we say hands-on, it's truly hands-on, no-demo. "1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay", Next batch 20-26 October. For details, click on: http://icsccb.org/workshops/cellcultureworkshop/

Following techniques will be done hands-on by each candidate using HEK-293T and Jurkat cell lines:
1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This is designed for beginners who wants to learn from scratch. This is a real hands-on workshop to teach and train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human/cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Cell Culture Workshop | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Cell Culture Workshop 1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, New Delhi, India Venue: ICSCCB, First Floor, Khasra 132, Plot 4, IGNOU Main Road, Near Saint Robin Public School, Neb Sarai, Saket, New Delhi – 110068, India (Nearest Metro Station is Saket on the Y...

"1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay", 24-30 December. For details, click on: http://icsccb.org/workshops/cellcultureworkshop/

ALL HANDS-ON
1. Cell culture techniques, passaging and maintenance of human cancer cell lines
2. Media preparation
3. Cryopreservation: Freezing & thawing of human cancer cell lines
4. Genomic DNA isolation from cancer cell lines,
5. Genomic DNA estimation
6. Genomic DNA agarose gel electrophoresis
7. Total RNA extraction from human cancer cell lines
8. cDNA synthesis from RNA (Reverse Transcription),
9. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
10. Agarose gel electrophoresis of PCR product
11. Total protein extraction from human cancer cell lines
12. Protein estimation
13. SDS-PAGE electrophoresis of extracted proteins
14. Coomassie blue staining of protein gel
15. MTT Assay for cell proliferation/cytotoxicity

This is designed for beginners who wants to learn from scratch. This is a real hands-on workshop to teach and train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human/cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Cell Culture Workshop | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Cell Culture Workshop 1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, New Delhi, India Venue: ICSCCB, First Floor, Khasra 132, Plot 4, IGNOU Main Road, Near Saint Robin Public School, Neb Sarai, Saket, New Delhi – 110068, India (Nearest Metro Station is Saket on the Y...

Tune in tomorrow morning at 09:15 AM for this conversation! (On 27.06.2023)

Visited All India Radio - Akashvani Bhavan, New Delhi along with Dr. Poonam Singh.

Don't invest in Pyramid schemes! Do a google search on MLM and pyramid schemes and educate yourself and your friends and relatives. Lots of people in India are losing money in these schemes and still new members are ready to join to lose their money!

How to Spot a Pyramid Scheme Don’t confuse a pyramid scheme for a legitimate opportunity.See The American Dream Denied: Herbalife Victims Speak Out: https://www.youtube.com/watch?v=oNM3M...

1-Month Biotech, Animal Cell Culture, Cancer Biology Summer Training, Fees Rs. 19500, starting on 1 May 2023. For details & payment of fees please visit our website: http://www.icsccb.org/summer-training/

Details of techniques (hands-on) to be learnt during the program:

Molecular Biology and Cell Culture Lab:
1. Restriction digestion
2. Ligation
3. DNA gel electrophoresis
4. DNA gel extraction
5. Preparation of competent cells
6. Transformation and plating on LBA plates
7. Plasmid DNA isolation
8. Genomic DNA isolation
9. Polymerase Chain Reaction (PCR)
10. Animal Cell Culture and maintenance of human cancer cell lines
11. Cryopresrvation: Freezing & thawing of human cancer cell lines
12. RNA extraction from human cancer cell lines
13. Reverse Transcription (cDNA synthesis)
14. Reverse Transcription PCR (RT-PCR)
15. Induction of proliferation and differentiation in human cancer cell lines
16. Protein extraction from human cancer cell lines
17. SDS-PAGE and coomassie blue staining
18. MTT Assay for proliferation/cytotoxicity
19. Cytospin slide preparation and staining of hematopoietic cells for visualizing differentiation of cells

Bioinformatics Lab:
20. DNA sequence analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
21. Protein sequence analysis
22. Protein structure visualization
23. Bioinformatics databases
24. PCR primer designing
25. Gene cloning
26. Promoter analysis
27. Whole genome data visualisation: browsing human and mouse genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

Summer Training | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Summer Training Biotech and Animal Cell Culture Summer Training, at New Delhi (1 Month, Full-time, Regular Program) Number of seats: 12 Next batch starting on: 1 May, 1 June, and 1 July 2023 Venue: ICSCCB, Kh. No. 132, Plot 4, IGNOU Main Road, opposite Hero Motors Service Centre, Neb Sarai, New Delh...

1-Month Biotech and Animal Cell Culture Summer Training, Fees Rs. 19500, 1 May, 1 June, 1 July 2023.

For details and registration please click on the following website:
http://www.icsccb.org/summer-training/

Details of techniques (hands-on) to be learnt during the program:

Molecular Biology and Cell Culture Lab:
1. Restriction digestion
2. Ligation
3. DNA gel electrophoresis
4. DNA gel extraction
5. Preparation of competent cells
6. Transformation and plating on LBA plates
7. Plasmid DNA isolation
8. Genomic DNA isolation
9. Polymerase Chain Reaction (PCR)
10. Animal Cell Culture and maintenance of human cancer cell lines
11. Cryopresrvation: Freezing & thawing of human cancer cell lines
12. RNA extraction from human cancer cell lines
13. Reverse Transcription (cDNA synthesis)
14. Reverse Transcription PCR (RT-PCR)
15. Induction of proliferation and differentiation in human cancer cell lines
16. Protein extraction from human cancer cell lines
17. SDS-PAGE and coomassie blue staining
18. MTT Assay for proliferation/cytotoxicity
19. Cytospin slide preparation and staining of hematopoietic cells for visualizing differentiation of cells

Bioinformatics Lab:
20. DNA sequence analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
21. Protein sequence analysis
22. Protein structure visualization
23. Bioinformatics databases
24. PCR primer designing
25. Gene cloning
26. Promoter analysis
27. Whole genome data visualisation: browsing human and mouse genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

Summer Training | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Summer Training Biotech and Animal Cell Culture Summer Training, at New Delhi (1 Month, Full-time, Regular Program) Number of seats: 12 Next batch starting on: 1 May, 1 June, and 1 July 2023 Venue: ICSCCB, Kh. No. 132, Plot 4, IGNOU Main Road, opposite Hero Motors Service Centre, Neb Sarai, New Delh...

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques, Molecular Biology & MTT Assay, New Delhi, India. Fees Rs. 9500, 22-28 April, 22-28 May, 22-28 June, 22-28 July 2023.

For more dates and registration details please visit the following website: http://icsccb.org/workshops/cellcultureworkshop/

Following techniques will be covered using human embryonic kidney cell line and human blood cancer cell lines:
1. Basic cell culture techniques, passaging and maintenance of cell lines
2. Media preparation
3. Cryopreservation (freezing and thawing of cell lines)
4. Genomic DNA isolation from cancer cell lines,
5. DNA estimation
6. DNA agarose gel electrophoresis
7. Total RNA extraction
8. cDNA synthesis from RNA (Reverse Transcription),
9. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
10. Agarose gel electrophoresis of PCR product
11. Total protein extraction
12. Protein estimation
13. SDS-PAGE electrophoresis of extracted proteins
14. Coomassie staining of protein gel
15. MTT Assay for cell proliferation/cytotoxicity

This is a basic workshop intended for beginners to teach and train them in animal cell culture and molecular biology techniques using the human/cancer cell lines for various experiments. It will be supported by some lectures on basic cell culture techniques, media preparation, gene/protein expression & interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Cell Culture Workshop | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Cell Culture Workshop 1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, New Delhi, India Venue: ICSCCB, Kh. No. 132, Plot 4, IGNOU Main Road, opposite Hero Motors Service Centre, Neb Sarai, New Delhi – 110068, India (Closest Metro Station Saket on Yellow Line) Google ma...

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques, Molecular Biology & MTT Assay, New Delhi, India.

For dates and registration please click the following website: http://icsccb.org/workshops/cellcultureworkshop/

Following techniques will be covered using human embryonic kidney cell line and human blood cancer cell lines:
1. Basic cell culture techniques, passaging and maintenance of cell lines
2. Media preparation
3. Cryopreservation (freezing and thawing of cell lines)
4. Genomic DNA isolation from cancer cell lines,
5. DNA estimation
6. DNA agarose gel electrophoresis
7. Total RNA extraction
8. cDNA synthesis from RNA (Reverse Transcription),
9. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
10. Agarose gel electrophoresis of PCR product
11. Total protein extraction
12. Protein estimation
13. SDS-PAGE electrophoresis of extracted proteins
14. Coomassie staining of protein gel
15. MTT Assay for cell proliferation/cytotoxicity

This is a basic workshop intended for beginners to teach and train them in animal cell culture and molecular biology techniques using the human/cancer cell lines for various experiments. It will be supported by some lectures on basic cell culture techniques, media preparation, gene/protein expression & interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Cell Culture Workshop | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Cell Culture Workshop 1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, New Delhi, India Venue: ICSCCB, Kh. No. 132, Plot 4, IGNOU Main Road, opposite Hero Motors Service Centre, Neb Sarai, New Delhi – 110068, India (Closest Metro Station Saket on Yellow Line) Google ma...

Biotech and Animal Cell Culture Summer Training
For dates and registration please click on the following website:
http://www.icsccb.org/summer-training/

Details of techniques (hands-on) to be learnt during the program:

Molecular Biology and Cell Culture Lab:
1. Restriction digestion
2. Ligation
3. DNA gel electrophoresis
4. DNA gel extraction
5. Preparation of competent cells
6. Transformation and plating on LBA plates
7. Plasmid DNA isolation
8. Genomic DNA isolation
9. Polymerase Chain Reaction (PCR)
10. Animal Cell Culture and maintenance of human cancer cell lines
11. Cryopresrvation: Freezing & thawing of human cancer cell lines
12. RNA extraction from human cancer cell lines
13. Reverse Transcription (cDNA synthesis)
14. Reverse Transcription PCR (RT-PCR)
15. Induction of proliferation and differentiation in human cancer cell lines
16. Protein extraction from human cancer cell lines
17. SDS-PAGE and coomassie blue staining
18. MTT Assay for proliferation/cytotoxicity
19. Cytospin slide preparation and staining of hematopoietic cells for visualizing differentiation of cells

Bioinformatics Lab:
20. DNA sequence analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
21. Protein sequence analysis
22. Protein structure visualization
23. Bioinformatics databases
24. PCR primer designing
25. Gene cloning
26. Promoter analysis
27. Whole genome data visualisation: browsing human and mouse genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

Summer Training | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Summer Training Biotech and Animal Cell Culture Summer Training, at New Delhi (1 Month, Full-time, Regular Program) Number of seats: 12 Next batch starting on: 1 May, 1 June, and 1 July 2023 Venue: ICSCCB, Kh. No. 132, Plot 4, IGNOU Main Road, opposite Hero Motors Service Centre, Neb Sarai, New Delh...

Delivered a guest lecture at Buddha Institute of Pharmacy, Gorakhpur today.

अगर सब लोग "जी हुजूर, हाँ माई बाप, यस सर" टाइप के हो जाएंगे तो देश का क्या होगा? कुछ हमारे जैसे "सीधी बात नो बकवास" टाइप के भी होने चाहिए।

इंटरनेशनल ईयर ऑफ़ द मिलेट्स विकसित और धनी देशों के लिए अच्छा है जहाँ पर जनसँख्या कम है और अनाज का उत्पादन ज्यादा है। हमारे यहाँ अगर गेहूं और धान/मक्का कि जगह साँवाँ, कोदो, ज्वार और बाजरा पैदा करने लगे तो आधे लोग तो भूखे मर जाएंगे। याद है देश स्वतंत्र होने के बाद अनाज की कितनी कमी थी? तब उन्नत खेती और हाइब्रिड गेंहू, धान और मक्का वगैरह की किस्मों से उत्पादन बढ़ा और हमारा देश भुखमरी से बचा। जबकि तब हमारी जनसँख्या बहुत कम थी और अब तो बहुत ज्यादा है। अगर मोटे अनाज की पैदावार बढ़ानी ही है तो हमारा नारा यह होना चाहिए कि "तम्बाकू की खेती बंद करो और गन्ना की खेती कम करो, और इनकी जगह मोटे अनाज की खेती करो"। तम्बाकू से कैंसर होता है जो कि भारत में बहुत बढ़ रहा है और ज्यादा चीनी/मिठाई खाने से डायबिटीज बढ़ता है जो भारत को "डायबिटीज कैपिटल ऑफ़ दि वर्ल्ड" बना रहा है।

Understand the message in this video. इस वीडियो को देखिये और समझिये कि ये वोट के बारे में क्या सन्देश दे रही है।
https://www.youtube.com/watch?v=be2Z1nRYSeU

ELECTION | VOTE VS NOTE

We have decided to fight the 2024 Parliamentary Elections after discussing with my wife Dr. Poonam Singh. This is an announcement of our intention. Which party or which parliamentary constituency would be decided in future after more discussions and consultations with other well-wishers.

Political parties who wants a fresh face with honest intentions to serve the country may contact me for discussions if they want to give me a ticket.

More to come at later dates in future!

"The Union Finance Secretary's comments on budgetary allocation for education in his interview to the daily reflect his lack of proper understanding on matters concerning education and are highly irresponsible," the ABVP said.

"Such remarks will only boost mistrust against the government in the education community, at a time when the entire country is anticipating substantial changes after the New Education Policy. The remarks made by the Union Finance Secretary regarding the education sector are insensitive and unfortunate," ABVP's National General Secretary Yagyavalkya Shukla said.

https://www.ndtv.com/india-news/bjp-linked-student-body-slams-finance-secretarys-education-budget-comment-3771912

BJP-Linked Student Body Slams Finance Secretary's Education Budget Comment "Pushing more money into education will achieve nothing," Finance Secretary TV Somanathan had said in an interview last week.

This is the kind of thinking which are destroying our education system. India will never become "vishwa guru" with this attitude towards education/higher education.
https://thewire.in/economy/pushing-more-money-into-education-will-achieve-nothing-finance-secretary

'Pushing More Money into Education Will Achieve Nothing': Finance Secretary Even for higher education, according to the finance secretary, pushing more funding will only be "a sop to the conscience of the intelligentsia that we are doing something for it".

जो लोग अपने वोट और सपोर्ट के बदले में पैसा चाहते हैं, तेल/पानी/नाश्ता का खर्चा चाहते हैं वो कृपया हमसे दूर रहें, इस इलेक्शन में मुझे ऐसे बहुत सारे लोगों ने कांटेक्ट किया, सपोर्ट किया और घुमा फिरा के लास्ट में पैसे पर ही आ गए। बहुत सारी ऐसी कहानिया हैं जिनको धीर धीरे लिखूंगा।

ईमानदारी से इलेक्शन लड़ने के लिए हमें खुद पैसे और मदद की जरुरत होती है। जो हमारे जेन्युइन समर्थक हैं जिनको अपने अंदर से आवाज आती है की साफ़ सुथरी राजनीति भी की जा सकती है और जो ऐसे लोगों को राजनीति में आते हुए देखना चाहते हैं और नि:स्वार्थ मदद करना चाहते हैं उनका भरपूर स्वागत है।

जिनको हमारी इतनी सीधी बात भी समझ में नहीं आ रही है वो भी हमसे दूर ही रहें।

बहुत बहुत धन्यवाद।

ये आर्टिकल पूरा पढ़िए (लिंक पर क्लिक करके खोलिये)। बहुत अच्छी तरह से एक्सप्लेन किया है, वैरी वेल रिटेन।
https://theprint.in/opinion/bbcs-anti-modi-stance-promotes-imagined-hindu-supremacy-muslims-in-india-are-not-at-risk/1344940/

BBC’s anti-Modi stance promotes imagined Hindu supremacy. Muslims in India are not at risk My Leftist friends want to believe that Modi is a bad guy. The BBC, of course, has listened to them and is convinced that Modi is a bad guy too.

जिन साथी ग्रेजुएट्स ने मतदान किया उनको बहुत बहुत धन्यवाद (चाहे जिसको भी किया)। किसी भी लोकतंत्र में वोट देना बहुत महत्वपूर्ण है। इस बार मत प्रतिशत बहुत कम था उम्मीद है कि भविष्य में और ज्यादा लोग वोटर रजिस्ट्रेशन करेंगे और वोट भी करेंगे। फाइनल रिजल्ट्स नीचे अटैच्ड है:

नर हो, न निराश करो मन को

कुछ काम करो, कुछ काम करो
जग में रह कर कुछ नाम करो
यह जन्म हुआ किस अर्थ अहो
समझो जिसमें यह व्यर्थ न हो
कुछ तो उपयुक्त करो तन को
नर हो, न निराश करो मन को

संभलों कि सुयोग न जाय चला
कब व्यर्थ हुआ सदुपाय भला
समझो जग को न निरा सपना
पथ आप प्रशस्त करो अपना
अखिलेश्वर है अवलंबन को
नर हो, न निराश करो मन को

जब प्राप्त तुम्हें सब तत्त्व यहाँ
फिर जा सकता वह सत्त्व कहाँ
तुम स्वत्त्व सुधा रस पान करो
उठके अमरत्व विधान करो
दवरूप रहो भव कानन को
नर हो न निराश करो मन को

निज़ गौरव का नित ज्ञान रहे
हम भी कुछ हैं यह ध्यान रहे
मरणोंत्‍तर गुंजित गान रहे
सब जाय अभी पर मान रहे
कुछ हो न तज़ो निज साधन को
नर हो, न निराश करो मन को

प्रभु ने तुमको दान किए
सब वांछित वस्तु विधान किए
तुम प्राप्‍त करो उनको न अहो
फिर है यह किसका दोष कहो
समझो न अलभ्य किसी धन को
नर हो, न निराश करो मन को

किस गौरव के तुम योग्य नहीं
कब कौन तुम्हें सुख भोग्य नहीं
जान हो तुम भी जगदीश्वर के
सब है जिसके अपने घर के
फिर दुर्लभ क्या उसके जन को
नर हो, न निराश करो मन को

करके विधि वाद न खेद करो
निज़ लक्ष्य निरन्तर भेद करो
बनता बस उद्‌यम ही विधि है
मिलती जिससे सुख की निधि है
समझो धिक् निष्क्रिय जीवन को
नर हो, न निराश करो मन को
कुछ काम करो, कुछ काम करो

आज हमारी शादी की 25वीं सालगिरह है लेकिन हम लोग वोट काउंटिंग की मीटिंग अटेंड करके 15 मतगणना एजेंट की आइडेंटिटी कार्ड बनवाये और उसी में रात के 8 बज गए। कल सुबह 8 बजे से मतगणना शुरू होगी। अब देखते हैं आगे क्या होगा। डॉ. पूनम सिंह के साथ।

जिला वाइज वोटिंग प्रतिशत एवं नंबर, गोरखपुर-फैज़ाबाद स्नातक क्षेत्र चुनाव 30.01.2023
साथी ग्रेजुएट्स जिन्होंने पोलिंग बूथ तक जाकर मतदान किया उनको बहुत बहुत धन्यवाद।

डॉ. शिव मोहन सिंह, प्रत्याशी, गोरखपुर-फैज़ाबाद खंड स्नातक विधायक (एम.एल.सी.) चुनाव 2023. बीएससी (कृषि एवं पशुपालन) कानपुर, एमएससी (इंग्लैंड), पीएचडी (जर्मनी), पीडीऍफ़ (अमेरिका, जर्मनी, इंग्लैंड)।

गोरखपुर-फैजाबाद खंड स्नातक निर्वाचन(एमएलसी चुनाव) के लिए सोमवार को महज 43.19 फीसदी मतदाताओं ने ही अपने मताधिकार का इस्तेमाल किया। गोरखपुर में सबसे कम 33.83 फीसदी वोटिंग हुई जबकि सबसे अधिक 45865 वोटर यहीं थे। अकेले बलरामपुर के मतदाता प्रथम श्रेणी में पास हुए। यहां सबसे अधिक 60.09 फीसदी वोट पड़े। मतदान शांतिपूर्ण माहौल में संपन्न हुआ।
https://www.amarujala.com/photo-gallery/gorakhpur/voting-begins-for-graduate-seat-in-gorakhpur-basti-division-2023-01-30?pageId=1

गोरखपुर-फैजाबाद स्नातक निर्वाचन: 43.19 फीसदी ने ही किया मतदान, पिछले चुनाव से कम हुई वोटिंग गोरखपुर-फैजाबाद खंड स्नातक निर्वाचन क्षेत्र के एमएलसी चुनाव के लिए मतदान सोमवार सुबह आठ बजे से शुरू हो गया है, जो श....