Promega Benelux

How are our customers using Maxwell® for cell and gene therapy?

• Biodistribution studies for adeno-associated virus (AAV) or lentivirus vector therapy development
• Viral genome titer assays in the preclinical phase and/or QC batch release processes to ensure the quality and consistency of lentiviral vectors
• In biomarker discovery, gene expression profiling or functional studies to analyse mRNA expression
• In vector integration analysis, safety and efficacy evaluation or monitoring gene therapy outcomes to look at transgene copy number
• DNA purification to support the development, study of efficacy and manufacturing of CAR-T cell therapies.

Find out more about the Maxwell and its Automated Nucleic Acid Extraction for Cell and Gene Therapy Applications at https://bit.ly/3UaFnUc

Improve the Quality and Consistency of Extracted Nucleic Acid for your Cell and Gene Therapy Application

The cell and gene therapy landscape is broad and complex, however a common theme is that many workflows require the extraction of nucleic acids. Promega’s Maxwell® system is proving a popular solution for this purpose.

Maxwell® is an automated, benchtop nucleic acid extraction platform that extracts high quality DNA, RNA (including mRNA and miRNA) and viral TNA (total nucleic acid) from a wide variety of cells, tissues and biological fluids to produce nucleic acid of a high yield and quality. Automating the extraction process has a number of advantages over manual methods such as ensuring operator-to-operator consistency, which is a pre-requisite for robust data production.

Learn more at: https://bit.ly/4cMOvFM

[𝐍𝐞𝐰 𝐏𝐫𝐨𝐝𝐮𝐜𝐭]
🌟 Elevate Your Research with 𝙈𝙚𝙩𝙖𝙗𝙤𝙡𝙞𝙩𝙚-𝙂𝙡𝙤™ Detection System! 🔬

💡 Achieve precise quantification with our bioluminescent detection system, compatible with various samples including cultured cells and serum. This toolkit simplifies your workflow through an easy, add-and-read protocol, ideal for high-throughput applications.

🚀 Perfect for those in drug discovery and metabolic research, the Metabolite-Glo™ Detection System is your key to efficient, scalable assays. Don't let complex preparation slow you down; our system is designed for the fast-paced demands of modern research.

🔗 Dive deeper and enhance your studies by visiting Promega's website: https://bit.ly/3xicHiS

[𝐓𝐞𝐜𝐡𝐧𝐨𝐥𝐨𝐠𝐲]
Looking for simple biochemical assays to measure protein-protein or protein-small molecule interactions involving tagged proteins?

🔆 Discover how Lumit can help you in this poster from Dr. Ellen Crummy, “Lumit™protein interaction immunoassays using protein tags and biotinylated small molecules”
https://bit.ly/3TJe7Mp

Join us this Wednesday, March 20th, for our webinar 'Innovative ADCC and ADCP Technologies and Services for Cell Therapies and Therapeutic Antibodies'. 💊

Our featured speakers, Julia Gilden from Promega and David Coberta from Charles River Laboratories will highlight technological advances in the field and share their experiences with innovative bioassays.

Learn about:
✔️ Implementing the HiBiT Target Cell Killing (TCK) platform for a sensitive, specific, and consistent read-out of effector cell function
✔️ Measuring ADCC and ADCP using HiBiT TCK Bioassays
✔️ Evaluating Fc-mediated antibody functions for early discovery programs

Register now ➡️ https://bit.ly/43moV6c

📆 20 March 2024
🕔 4pm CET

Our team is ready to welcome you at Laborama today and tomorrow.
Pass by our booth C7 to chat about our latest instruments and technological innovations.

[𝐁𝐥𝐨𝐠]
Are you interested in ?
Quantifying intracellular pyruvate is now easier then ever using Pyruvate-Glo™

Find out more in our latest blog where we discuss the current understanding of metabolism, it’s production and its disease implications.

Read more at: https://bit.ly/3v7F1DY

💡𝐆𝐞𝐭 𝐦𝐨𝐫𝐞 𝐝𝐚𝐭𝐚 𝐟𝐫𝐨𝐦 𝐲𝐨𝐮𝐫 𝐩𝐫𝐞𝐜𝐢𝐨𝐮𝐬 𝐞𝐱𝐩𝐞𝐫𝐢𝐦𝐞𝐧𝐭𝐬!

Lumit™ immunoassays multiplex effortlessly with cell viability and cytotoxicity assays, enabling you to get 3 data points from a single well.
✔️ Measure cytotoxicity over time using CellTox-Green
✔️ Collect supernatant for Cytokines quantitation using Lumit
✔️ Read cell viability using CellTiter-Glo

Not interested in cell viability? Harvest your cells for RNA extraction.

🤓 Read more
Lumit : https://bit.ly/48A9ZlW
Cell Health : https://bit.ly/3wMJyfu
RNA Extraction : Manual (https://bit.ly/3IpzonE) or Benchtop automated (https://bit.ly/48ECajF)

🧫 [𝐂𝐞𝐥𝐥𝐮𝐥𝐚𝐫 𝐌𝐞𝐭𝐚𝐛𝐨𝐥𝐢𝐬𝐦]
🔦 𝙎𝙝𝙞𝙣𝙚 𝙖 𝙡𝙞𝙜𝙝𝙩 𝙤𝙣 𝙬𝙝𝙖𝙩 𝙥𝙤𝙬𝙚𝙧𝙨 𝙮𝙤𝙪𝙧 𝙘𝙚𝙡𝙡𝙨 𝙪𝙨𝙞𝙣𝙜 𝙤𝙪𝙧 𝙢𝙚𝙩𝙖𝙗𝙤𝙡𝙞𝙨𝙢 𝙖𝙨𝙨𝙖𝙮𝙨 💪💥

Cellular energy metabolism is the consequence of dynamic interplay between many metabolic pathways. Every cell has a metabolic profile, and each metabolite plays a particular role in regulating energy levels.

Promega offers sensitive, easy-to-use bioluminescent assays to monitor metabolic pathways and a variety of related factors. Explore them here ➡️ https://bit.ly/431BvI0

⏲️ Fast, Reliable Alternative to 's : Lumit™

✅ Lumit™ Immunoassays is an "Add-Mix-Measure" solution, eliminating the variability associated with multiple wash steps, and providing the convenience of a simple and sensitive luminescent detection method.

🦾Lumit™ Immunoassays are easily adaptable to automation for labs processing large numbers of samples.

More at: https://bit.ly/3UJfCuM

UPDATE: we are back online ❤️

Our website is currently down, and we are working on a fix. Meanwhile feel free to call us for product or price information: +31715324244 Happy Monday!

If you want to join today’s online Discover Glo scientific conference, simply visit www.promega.com/discoverglo Participation is free, and you will enjoy presentations given by scientist from France, the Netherlands, UK and the US.

Lumie is on her way to Antwerp for Promega's 2023 tomorrow! There's still time to register and join this free event online to hear from leading researchers and great speakers at https://bit.ly/3LpsyAQ

At this year (Tuesday. 25 April 2023) Anne Jackson, PhD, AstraZeneca will present her talk - studying compound binding kinetics using NanoBRET distinguishes between highly similar targets in cells.

Compound selectivity between targets and off-targets is often usefully measured by comparing compound potency at equilibrium, but this does not always give the whole picture. When investigating the inhibition of two highly similar kinases, these measures at equilibrium showed little selectivity across a range of biochemical and cellular systems. When biophysical methods showed differences in binding kinetics, they investigated the compound behaviour in cells using NanoBRET target engagement assays in kinetic mode. These showed dramatic differences in rates of binding and the length of compound-target interaction between the two kinases, suggesting greater selectivity than previously suspected from the equilibrium data and informing the design of subsequent experiments to factor in the time-dependent nature of the compound activity. The impacts of these findings and learnings from generating and analysing large amounts of kinetic data will be discussed, along with the importance of understanding the assumptions of biochemical models when applying them to cellular settings.

Dr Anne Jackson joined AstraZeneca in 2017 as a Research Scientist and later a Senior Research Scientist within the Mechanistic Biology and Profiling Department. She has expertise in a range of high-throughput biochemical and cellular assays, and especially enjoys combining the two disciplines to look at in-cell kinetics, using these insights to impact a wide range of oncology projects.

For full agenda and free register at (online or in -person in Antwerp) look at https://bit.ly/41Etbf6

Have you registered yet for 2023 - Biologically Relevant Models to Advance your Research - on 25 April 2023?

There are some great speakers lined up during a full day of scientific talks delivered by renowned international speakers from across Promega and its customer network.

If you can’t make the in-person event, in Antwerp, Belgium you can join us online for the day.

Take a look and register for free at: https://bit.ly/40mMlFv

Integrating bioluminescence and microfluidic chips for single-cell imaging and multiscale analysis is the title of Giorgia Zambito. PhD (Erasmus MC Rotterdam) talk at this year's on 25 April 2023 in Belgium or online.

Giorgia Zambito is a postdoc researcher with a strong background in pre-clinical research, with a specialisation in optical molecular imaging.

Incorporating non-invasive biosensing features in organ-on-chip models is of paramount importance for a wider implementation of these advanced in vitro microfluidic platforms. Optical biosensors, based on Bioluminescence Imaging (BLI), enable continuous, non-invasive, and in-situ imaging of cells, tissues or miniaturised organs without the drawbacks of conventional fluorescence imaging. Bioluminescence has great promise as a highly desirable biosensor for studying organ on chip platforms.

Discover more and/or register at: https://bit.ly/3mGpua9

From Petri Dishes to Chips: How Biochemical Assays Enhance Organ-on-Chip Technology is the title of Flavio Bonanini's talk at this year's (which is on Tuesday, 25 April 2023 in Antwerp, Belgium or online).

From 2019, Flavio has been a Marie Sklodowska-Curie fellow student affiliated with MIMETAS, a biotech company located in Leiden, Netherlands, as part of the PoLiMeR consortium. His current focus is on liver models and contributes to the development of a throughput-capable organ-on-chip culture platform, which MIMETAS specialises in developing and commercialising.

The successful translation of pre-clinical research results into clinical practice remains a significant challenge for the pharmaceutical industry. Clinical trials often fail, have limited efficacy, or induce toxic side effects that were not anticipated. This is in part due to inadequate pre-clinical models, such as two-dimensional cell cultures, which do not mimic the complex environment cells find in vivo. Recent advances in organ-on-a-chip technology offer a promising avenue to model important aspects of human physiology in vitro.

MIMETAS' OrganoPlate® is an organ-on-a-chip platform that allows for controlled and complex cultivation of cellular systems in three dimensions, mimicking architecture, mechanical properties, cellular composition and biochemical functionalities of healthy and diseased organs. The integration of organ-on-a-chip technology and reliable assays could transform pre-clinical drug development by providing accurate and predictive models of human physiology.

Discover more during this year's Discover Glo. There are a few places left you will need to book on our website to secure a place at: https://bit.ly/3UJoGOi

Heading to in Copenhagen April 15-18? Visit us in booth B4-08 to learn about our catalog and custom solutions to advance your infectious disease research from made-to-order master mixes and wastewater surveillance tools to genomic DNA extraction and PCR amplification assays. In the meantime, preview our featured solutions. We look forward to connecting soon! Click to learn what’s new: http://bit.ly/3TXb4P8

Join Andrew L. Niles from Promega Corporation online or in person at this year's Discover Glo for his talk Exploring Endogenous Cell Biology with CRISPR-Mediated Bioluminescent Tagging commencing 9.45am (BST) on Tuesday, 25 April 2023.

Andrew L. Niles is a Senior Research Scientist in Promega’s Advanced Technology Group. His scientific focus is directed at providing innovative solutions for improving in vitro gene editing efficiencies allowing for integration of bioluminescent tags and reporters.

Stably integrated transgenes and tags have been useful for interrogating basic aspects of cell biology, but often fail to fully reflect physiologically relevant parameters relating to binding partners and interactors. These known limitations may adversely skew data set interpretation. CRISPR-mediated methods now allow for specific and precise incorporation of tags into native loci (“knockins”), thus offering notable and incremental improvement in achieving true endogenous biology.

This seminar describes our efforts to improve gene editing efficiencies and workflows, means to identify and characterise those edits, and to demonstrate their utility using well-validated cancer pathway protein:protein interactions. Specific emphasis will be focused on our luciferase and luciferase complementation tagging systems and the relative merits or limitations of each approach.

Discover more and reserve your place at: https://bit.ly/3KuhcKd

The Promega Team wishes you a Happy Easter!

Our office will be closed on Monday 10 April. Enjoy your long weekend!

🐰🐣 Happy Friday, egg hunters! This is the last day of our online egg hunt, and it's your last chance to find the hidden egg on our website! 🕵️‍♀️🔎

To give you a hint, here's today's riddle: "I'm the immunoassay with a rapid pace, Skipping washes without a trace. What am I?" 🤔

Remember, the daily prize is still up for grabs! 🎁🎉

www.promega.com/egghunt

🐰🐣 Hello egg hunters! Day number four in our online egg hunt! 🕵️‍♀️🔎 Did you manage to find the previous eggs?? 🍀 grab your new chance today!

To give you a hint, here's today's riddle: "I'm the powerful tool for clinical extraction, Automating nucleic acids with precision. What am I?" 🤔 Keep searching our website for the hidden egg and use the riddle to help you find it!

Www.promega.com/egghunt

🐰🐣 Rise and shine, hunters! It's another day of our online egg hunt! 🕵️‍♀️🔎

Starting today until Friday, join us in the search for the hidden egg on our website! Here's today's riddle to help you find it: "I'm a scientific tool with precision and flair, Sequencing and analyzing DNA fragments with care
, what am I?" 🤔

🎁🎉 Keep searching and don't miss out on the chance to win! 🥚👀

Www.promega.com/egghunt

And the Egg Hunt continues!! Today’s riddle is:

I'm the process that powers your life, Turning food into energy, free from strife.

Look for the hidden Easter Egg, we draw 2 two daily winners who win a box of chocolate eggs 🐣🐰

Www.promega.com/egghunt

🐣 Hop, hop, hop! The egg hunt is on! 🐰🌷

Starting today till Friday, join our online egg hunt and search for the hidden egg on our website. 🕵️‍♀️🔍

To help you find it, here's a riddle: "I glow in the dark, a machine of light, Use me to measure secrets, hidden from sight." 🤔

Every day, we'll pick two lucky winners who find
the egg and they'll win a fun prize! 🎉🎁

So, get cracking and start hunting! 🥚👀

Learn more: www.promega.com/egghunt

🎉🥚 Get ready for an egg-citing challenge, crafted exclusively for science enthusiasts! Join us in the Promega Easter Egg Hunt starting from Monday 27 March 2023. 🐣🔬🧪

👀 Follow us on social media to stay tuned and participate in this egg-straordinary challenge. 📲 We've got fun prizes for daily winners, so don't miss out on the chance to win big! 🏆

🐰🌷

Want to get live-cell kinetic data without an expensive microplate reader? Learn how to get great 24-hour or multi-day kinetic data using GloMax® plate readers.

How to Get Real-Time Kinetic Data With GloMax® Microplate Readers You don't need an expensive plate reader to get great real-time kinetic data. These videos show you how!

Is cell proliferation an important measure for your ongoing research? This Biocompare article discusses the many types of proliferation assays available and when to use them. https://bit.ly/3GmgfSb

🎄🎅🏻⛄️🌟 Happy holidays! As a reminder, our offices are closed between Christmas and New Year.

Wishing you a magical Winter Solstice, and many bright days to come 🌞🌟