Creative proteomics

Ketogenic Diet's Impact on Cancer Metabolism Revealed

Exciting new research has uncovered how a ketogenic diet can reshape cancer metabolism through lysine β-hydroxybutyrylation (Kbhb). Our multi-omics analysis of mouse livers shows that Kbhb is significantly influenced by the ketogenic diet. By combining bioinformatics with experimental validation, we've identified the critical role of Kbhb sites in modulating mTOR signaling and cancer cell metabolism.

This discovery could pave the way for innovative cancer therapies targeting metabolic pathways.

Read the full study here: https://www.nature.com/articles/s42255-024-01093-w

Ketogenic diet reshapes cancer metabolism through lysine β-hydroxybutyrylation - Nature Metabolism Multi-omics analysis of mouse livers shows that protein β-hydroxybutyrylation (Kbhb) is strongly affected by the ketogenic diet. Combining bioinformatics with experimental validation, a role of Kbhb sites in modulating mammalian target of rapamycin signalling and cancer cell metabolism is identifie...

Maternal Liver's Vital Role in Fetal Brain Development

Did you know that fetal brain development depends on the maternal supply of long chain polyunsaturated fatty acids (LC-PUFAs)? Our latest research uncovers a coordinated transcriptional program in the maternal liver, driven by pregnancy-induced liver-X-receptor (LXR) signaling. This process promotes the synthesis and export of LC-PUFA-containing phospholipids, ensuring the fetus receives the necessary nutrients for brain development.

This study sheds light on the crucial role of the maternal liver in supporting the developing brain during pregnancy.

Discover the full findings here:

A co-ordinated transcriptional programme in the maternal liver supplies long chain polyunsaturated fatty acids to the conceptus using phospholipids - Nature Communications Fetal brain development is dependent on the maternal supply of long chain polyunsaturated fatty acids (LC-PUFAs). Here, the authors show that pregnancy-induced liver-X-receptor (LXR) signaling in the maternal liver promotes the synthesis of LC-PUFA-containing phospholipids for export to the fetus.

Exploring the Hidden Role of Interleukin-1α: Proximity Labelling Reveals Conserved Nuclear Interactions

Interleukin-1α (IL-1α) is essential in inflammation, but its intracellular role has been a mystery. Our recent study employs proximity labelling to assess the interactome of pro-IL-1α, unveiling a conserved nuclear interaction with significant implications for immunobiology and evolutionary studies.

This research opens new avenues for understanding the intricate mechanisms of IL-1α within cells and highlights the evolutionary convergence and divergence of immune functions across species.

Read more about these findings and their impact on immunology and evolution.
https://www.nature.com/articles/s41467-024-50901-0

Proximity labelling of pro-interleukin-1α reveals evolutionary conserved nuclear interactions - Nature Communications Interleukin (IL)-1α is a critical inflammatory cytokine and yet its intracellular role is poorly understood. Here, the authors use proximity labelling of pro-IL-1α and assess its interactome, and dissect the presence and impact on a conserved nuclear interaction and its impact on the immunobiology...

Transform Your Research with Creative Proteomics' Luminex Cytokine Panel Service! 🌟

At Creative Proteomics, we are proud to offer the Luminex Cytokine Panel Service, leveraging state-of-the-art Luminex xMAP technology for high-throughput, simultaneous quantification of multiple cytokines. Our platform is designed for a wide range of species and is perfect for immune profiling, disease research, and biomarker discovery.

✨ Key Benefits:

Detect up to 100 analytes in one run
High sensitivity with detection limits as low as 0.06 pg/mL
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Whether you are conducting basic research or advanced clinical studies, our Luminex Cytokine Panel Service delivers the precision and efficiency you need.
https://cytokine.creative-proteomics.com/cytokine-panel-service.htm

Predicting glycan structure from tandem mass spectrometry via deep learning

CandyCrunch is a deep learning-based tool for predicting glycan structures from tandem mass spectrometry data. The paper also introduces CandyCrumbs that automatically annotates fragment ions in higher-order tandem mass spectrometry spectra.

Predicting glycan structure from tandem mass spectrometry via deep learning - Nature Methods CandyCrunch is a deep learning-based tool for predicting glycan structures from tandem mass spectrometry data. The paper also introduces CandyCrumbs that automatically annotates fragment ions in higher-order tandem mass spectrometry spectra.

Targeting fatty acid oxidation enhances response to HER2-targeted therapy

Metabolic reprogramming is crucial in tumorigenesis, with alterations in glucose and fatty acid metabolism playing key roles. Here, the authors show that inhibiting fatty acid oxidation in HER2-driven breast cancers delays tumor growth and enhances the effectiveness of HER2-targeted therapies.

Targeting fatty acid oxidation enhances response to HER2-targeted therapy - Nature Communications Metabolic reprogramming is crucial in tumorigenesis, with alterations in glucose and fatty acid metabolism playing key roles. Here, the authors show that inhibiting fatty acid oxidation in HER2-driven breast cancers delays tumor growth and enhances the effectiveness of HER2-targeted therapies.

Proteomic landscape of epithelial ovarian cancer

It remains essential to find clinically relevant biomarkers in epithelial ovarian cancer (EOC). Here, the authors perform a comprehensive proteomic profiling of tissue and plasma samples from EOC and control patients; they find potential biomarkers for EOC early detection and develop methods for tumour recurrence prediction.

Proteomic landscape of epithelial ovarian cancer - Nature Communications It remains essential to find clinically relevant biomarkers in epithelial ovarian cancer (EOC). Here, the authors perform a comprehensive proteomic profiling of tissue and plasma samples from EOC and control patients; they find potential biomarkers for EOC early detection and develop methods for tum...

🌟 Unlock Metabolic Insights with Creative Proteomics! 🌟

Discover the power of targeted metabolomics to delve into the biochemical complexities of life. Our advanced technologies, including GC/MS, LC/MS, HPLC/MS, and NMR, provide detailed analysis of key metabolic pathways and compounds. From glycolysis to neurotransmitters, our services cover a wide range of metabolites, offering tailored solutions for your research.

With high-throughput capabilities and rigorous quality control, Creative Proteomics is your partner in exploring the dynamic world of metabolism. Whether you're working with plant, animal, or clinical samples, we deliver precise and reliable results.

Join us in the journey to understand metabolism like never before!


https://www.creative-proteomics.com/services/targeted-metabolomics.htm

Curious about the differences between Discovery Proteomics and Targeted Proteomics? Our new infographic provides a clear comparison!

Discovery Proteomics focuses on broad protein screening and dynamics, utilizing techniques like SWATH and MSX-DIA. On the other hand, Targeted Proteomics is designed for the precise detection and absolute quantification of specific target proteins/peptides using methods such as SRM/MRM and PRM.

The infographic includes:

Detailed workflows for each technique
Key characteristics and applications
Want to dive deeper? Download the PDF to learn more!
https://www.creative-proteomics.com/casedown-32-targeted-proteomics-vs-discovery-proteomics.htm

Multiomic profiling of medulloblastoma reveals subtype-specific targetable alterations at the proteome and N-glycan level

Medulloblastomas (MBs) are highly heterogeneous paediatric brain tumours that remain challenging to treat. Here, the authors integrate proteomics, epigenomics, transcriptomics and post-translational modification analyses to find molecular subgroups and potential therapeutic targets in MB tumours.

Multiomic profiling of medulloblastoma reveals subtype-specific targetable alterations at the proteome and N-glycan level - Nature Communications Medulloblastomas (MBs) are highly heterogeneous paediatric brain tumours that remain challenging to treat. Here, the authors integrate proteomics, epigenomics, transcriptomics and post-translational modification analyses to find molecular subgroups and potential therapeutic targets in MB tumours.

Paternal microbiome perturbations affect offspring outcomes

Paternal origins of health and disease is an emerging paradigm highlighting the influence of paternal environmental exposures, including diet and lifestyle, on offspring health. The influence of the paternal gut microbiota on the germ line and offspring outcomes has been investigated in a new study.

Paternal microbiome perturbations affect offspring outcomes - Nature Reviews Urology Paternal origins of health and disease is an emerging paradigm highlighting the influence of paternal environmental exposures, including diet and lifestyle, on offspring health. The influence of the paternal gut microbiota on the germ line and offspring outcomes has been investigated in a new study.

🌟 Revolutionizing Cancer Treatment with ADC Therapies! 🌟
Antibody-Drug Conjugates (ADCs) are transforming the landscape of oncology by selectively delivering powerful drugs directly to cancer cells. Our latest brochure delves into the intricate mechanisms, market growth, and cutting-edge analytical methods behind these therapies. Join us in exploring how ADCs are enhancing treatment efficacy and minimizing toxicity. https://www.creative-proteomics.com/pronalyse/resource-overview-adc-therapies.html

Creative Proteomics is your partner in ensuring the quality and safety of biopharmaceutical products.
Our protein and antibody characterization services provide in-depth analysis of structure, purity, bioactivity, and other critical properties, adhering to ICH Q6B guidelines. We support a variety of biologics, including glycoproteins, monoclonal antibodies, and biosimilars. With our world-class mass spectrometry technology, we help you achieve success in your drug development journey. https://www.creative-proteomics.com/pronalyse/incl/pdf/protein-antibody-characterization-in-biopharmaceuticals-org.pdf

Spatially and temporally probing distinctive glycerophospholipid alterations in Alzheimer’s disease mouse brain via high-resolution ion mobility-enabled sn-position resolved lipidomics

Dysregulated glycerophospholipid metabolism in the brain is associated with neurodegenerative diseases. Here, the authors present a 4D lipidomics strategy that profiles glycerophospholipids at the sn-position-resolved level, revealing potentially altered remodeling pathways.

Spatially and temporally probing distinctive glycerophospholipid alterations in Alzheimer’s disease mouse brain via high-resolution ion mobility-enabled sn-position resolved lipidomics - Nature Communications Dysregulated glycerophospholipid metabolism in the brain is associated with neurodegenerative diseases. Here, the authors present a 4D lipidomics strategy that profiles glycerophospholipids at the sn-position-resolved level, revealing potentially altered remodeling pathways.

Diversity of post-translational modifications and cell signaling revealed by single cell and single organelle mass spectrometry

This study explored single cells and single organelles to reveal unique molecular characteristics such as mutant peptides and modulation of diverse post-translational modifications that are inaccessible through DNA/RNA profiling.

Diversity of post-translational modifications and cell signaling revealed by single cell and single organelle mass spectrometry - Communications Biology This study explored single cells and single organelles to reveal unique molecular characteristics such as mutant peptides and modulation of diverse post-translational modifications that are inaccessible through DNA/RNA profiling.

🌿 Assessment of the Tick Gut Metabolome Composition by Creative Proteomics 🌿

The gut microbiome plays a crucial role in regulating metabolic pathways and influencing host health. At Creative Proteomics, we offer untargeted metabolomics services to help you investigate the complex composition of intestinal microbial metabolites.

Check out our the case study where we detail how we've helped clients to analyze changes in intestinal metabolite composition, complete with experimental methods and results. Download for more insights!
https://www.creative-proteomics.com/casedown-1-assessment-of-the-tick-gut-metabolome-composition.htm

Explore the fascinating realm of protein characterization with Creative Proteomics! Our detailed overview covers essential techniques like molecular weight analysis, HPLC purity assessment, and in-depth post-translational modification studies. Discover how these analyses guarantee the quality and consistency of biopharmaceuticals. Read more: https://www.creative-proteomics.com/pronalyse/resource-protein-characterization-overview.html

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter

XylE is a bacterial xylose transporter and homologue of human glucose transporters GLUTs 1-4. HDX-MS, mutagenesis and MD simulations suggest that protonation of a conserved aspartate triggers conformational transition from outward- to inward facing state only in the presence of substrate xylose. In contrast, inhibitor glucose locks the transporter in the outward facing state.

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter - Nature Communications XylE is a bacterial xylose transporter and homologue of human glucose transporters GLUTs 1-4. HDX-MS, mutagenesis and MD simulations suggest that protonation of a conserved aspartate triggers conformational transition from outward- to inward facing state only in the presence of substrate xylose. In....

How bile acids and the microbiota interact to shape host immunity

Here, Raffatellu and co-workers discuss our growing understanding of how primary bile acids (which are cholesterol-derived molecules synthesized in the liver) and secondary bile acids (which are primary bile acids that have been microbially modified) shape immune responses in health and disease, with a particular focus on bile acids and intestinal immunity.

How bile acids and the microbiota interact to shape host immunity - Nature Reviews Immunology Here, Raffatellu and co-workers discuss our growing understanding of how primary bile acids (which are cholesterol-derived molecules synthesized in the liver) and secondary bile acids (which are primary bile acids that have been microbially modified) shape immune responses in health and disease, wit...

Elevate your protein analysis with Creative Proteomics' Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) service!

HDX-MS is essential for exploring protein conformation, dynamics, and interactions, crucial for biopharmaceutical development. Our advanced Orbitrap Exploris™ 240 Mass Spectrometer ensures high sensitivity and accuracy, with rapid turnaround times tailored to your needs. Discover the potential of HDX-MS for your projects today! Learn more: https://www.creative-proteomics.com/pronalyse/hydrogen-deuterium-exchange-mass-spectrometry-hdx-ms-service.html

Unlock the full potential of your biotherapeutic development with Creative Proteomics' Differential Scanning Calorimetry (DSC) service! Our cutting-edge DSC technology offers precise and efficient protein stability analysis, helping you identify optimal conditions and accelerate your drug development process. With high sensitivity and accuracy, our DSC service ensures reliable results and robust data analysis. Trust our expert team to provide customized solutions tailored to your research needs.
https://www.creative-proteomics.com/pronalyse/differential-scanning-calorimetry-dsc.html

Illuminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels

The molecular profiling of glycosphingolipids (GSLs) is hindered by the coexistence of abundant phospholipids and diverse isomers. The authors introduce a highly sensitive workflow that maps out the structural atlas of neutral GSLs, previously deemed a “dark space” within the lipidome.

Illuminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels - Nature Communications The molecular profiling of glycosphingolipids (GSLs) is hindered by the coexistence of abundant phospholipids and diverse isomers. The authors introduce a highly sensitive workflow that maps out the structural atlas of neutral GSLs, previously deemed a “dark space” within the lipidome.

Targeting metabolically activated fibroblasts in the failing heart

Myofibroblast activation requires nuclear translocation of ATP citrate lyase (ACLY) that triggers chromatin remodeling and the induction of fibrosis-associated genes. ACLY inhibition prevents myofibroblast conversion and causes de-differentiation of myofibroblasts to fibroblasts, indicating a potential therapeutic approach for heart failure.

Targeting metabolically activated fibroblasts in the failing heart - Nature Cardiovascular Research Myofibroblast activation requires nuclear translocation of ATP citrate lyase (ACLY) that triggers chromatin remodeling and the induction of fibrosis-associated genes. ACLY inhibition prevents myofibroblast conversion and causes de-differentiation of myofibroblasts to fibroblasts, indicating a potent...

Delve into Protein Complexes with TAP-MS at Creative Proteomics! 🧬

Explore the forefront of biological research with our Tandem Affinity Purification (TAP)-MS service. By harnessing cutting-edge mass spectrometry technology, we uncover intricate protein associations and interactions in their native environments. From experimental design to data analysis, trust Creative Proteomics for comprehensive insights into cellular functions and drug targets.
https://www.creative-proteomics.com/services/tandem-affinity-purification-tap-ms-service.htm

Discover the Power of Membrane Proteomics with Creative Proteomics! 🔬

Cell membrane proteins are essential for key functions like material exchange, cell recognition, immune response, signal transduction, regulation, and energy transfer. As many drugs target these proteins, understanding their characteristics and monitoring their expression is vital for new drug development.

Creative Proteomics offers specialized membrane proteomics solutions to enhance your research.

Our services include:
Protein Post-translational Modification Analysis
Protein-Protein Interaction Networks
Protein Identification
Protein Quantification
Download our infographic to learn more about our membrane proteomics solutions. Contact us for more information and see how we can support your research endeavors.
https://www.creative-proteomics.com/casedown-22-membrane-proteomics-solution.htm

Unveil the Mysteries of Glycans with Creative Proteomics! 🔬

Dive into the intricate world of glycobiology with our comprehensive Glycomics services. From glycan profiling to structural characterization, our state-of-the-art techniques like mass spectrometry and liquid chromatography ensure precise and insightful analysis. Whether you're exploring glycan modifications, glycoproteomics, or polysaccharide analysis, Creative Proteomics is your trusted partner in scientific discovery.

Join us in advancing the frontier of glycan research. Contact Creative Proteomics today to explore our Glycomics services!
https://www.creative-proteomics.com/services/glycomics-service.htm

Glycoproteomics

Glycoproteomics is coming of age, thanks to advances in instrumentation, experimental methodologies and computational search algorithms.

Glycoproteomics - Nature Methods Glycoproteomics is coming of age, thanks to advances in instrumentation, experimental methodologies and computational search algorithms.

Deep learning method for the prediction of glycan structures from mass spectrometry data

The annotation of structural glycomics data is a bottleneck in gaining insight into complex carbohydrates. An artificial intelligence model, CandyCrunch, has now been developed that accelerates the annotation process by orders of magnitude and achieves high accuracy in the prediction of glycan structures from tandem mass spectrometry data.

Deep learning method for the prediction of glycan structures from mass spectrometry data - Nature Methods The annotation of structural glycomics data is a bottleneck in gaining insight into complex carbohydrates. An artificial intelligence model, CandyCrunch, has now been developed that accelerates the annotation process by orders of magnitude and achieves high accuracy in the prediction of glycan struc...

Unlock the Power of Protein Interactions with Co-Immunoprecipitation Mass Spectrometry (Co-IP-MS)! 🔬

Discover how Co-IP-MS combines selective protein capture with high-resolution mass spectrometry to revolutionize protein interaction detection and characterization.

Why Choose Co-IP-MS?

Comprehensive Identification: Detects all proteins within a complex, including novel partners.
Quantifies Protein Interactions: Provides insights into interaction dynamics and stoichiometry.
Maps Interaction Networks: Reveals functional relationships through detailed networks.
No Prior Knowledge Required: Identifies all co-precipitated proteins for novel discoveries.
High Throughput: Efficiently studies complex biological processes.
Physiologically Relevant: Preserves native protein conformations.

Download the PDF to explore Co-IP-MS capabilities for your research. Contact us to learn more about how this advanced technology can benefit you!
https://www.creative-proteomics.com/casedown-62-co-immunoprecipitation-mass-spectrometry-platform.htm

Enhance Your Research with Creative Proteomics' TCA Cycle Analysis Service! 🔬

The TCA cycle is essential for energy production, metabolism, and biosynthesis. Our service offers precise and accurate analysis of TCA cycle metabolites, providing valuable insights into cellular functions and disease mechanisms.

With advanced LC-MS/MS techniques and experienced researchers, Creative Proteomics delivers reliable data for various applications, including clinical diseases, food industry, livestock, medicine, and environmental toxicology.

Explore the power of TCA cycle analysis with Creative Proteomics! https://www.creative-proteomics.com/services/tca-cycle-analysis-service.htm